Journal of Yeungnam Medical Science

Yung Chang Lee

4 Articles

Distribution patterns of cytoskelectal proteins in cardiac endothelial cells : Investigation using monoclonal antibodies.: Han Chul Kim, In Hwan Song, Yung Chang Lee; Yeungnam Univ J Med. 1990;7(2):27-37. Published online December 31, 1990; DOI: https://doi.org/10.12701/yujm.1990.7.2.27

Abstract PDF: To investigate the changing patterns of microfilament and microtubule arrangement and influence of myocardial cells and colchicines to microfilament and microtubule formation in cardiac endothelial cells the authors carried out indirect immunofluorescence stain for actin and tubulin with supernatant monoclonal antibodies. Secondary antibodies were IgG FITC conjugate. The results were summarized as follows. Fiberform reactions were stronger in the cells with many processes and spread cytoplasm and they became weaker after the endothelial cells formed monolayer. In the endothelial cells cocultured with myocardial cells the fiberform of the microtubule became less visible compared to control group but fiberform of the microtubule maintained strong intensity as endothelial cells formed monolayer. In the group treated with colchicines, there were no visible differences in microfilaments compared to control group but fiberform of microtubule revealed weaker intensity after colchicines treatment. The intensity of microtubule fiberform returned to control level after 2 days.

Transition of Marker Enzymes of Rat Hepatocyte Organelles in Culture.: In Hwan Song, Joo Yung Kim, Eon Ki Sung, Yung Chang Lee; Yeungnam Univ J Med. 1989;6(2):133-140. Published online December 31, 1989; DOI: https://doi.org/10.12701/yujm.1989.6.2.133

Abstract PDF: To investigate recovery, growth, and activity of hepatocyte in primary culture after cell separation, the authors followed up the marker enzyme activities of golgi complex, mitochondria and biologic membrane. Thiamine pyrophosphatase, the marker enzyme of golgi complex, activity approached the level of long term culture at 4th day. Succinate dehydrogenase, the marker enzyme of mitochondria, activity decreased with time, then it maintained constant level after 4th day. Alkaline phosphatase, the marker enzyme of biological membrane, activity increased from 3rd day, and after 5th day it showed strong reaction. These data suggested that hepatocytes were stabilized and recovered normal activity 4 day after cell separation. But the main secretory function was speculated to be reduced in culture.

Effects of Dimethyl Sulfoxide on the Differentiation of Myocardial and Endothelial Cells.: Dong Hyup Lee, Yee Tae Park, Sung Sae Han, Yung Chang Lee; Yeungnam Univ J Med. 1988;5(2):111-119. Published online December 31, 1988; DOI: https://doi.org/10.12701/yujm.1988.5.2.111

Abstract PDF: To elucidate the effects of dimethyl sulfoxide on of myocardial and endothelial cells in culture, the cells were exposed to 10% dimethyl sulfoxide in culture medium for 1 hour at 48 hours after cell isolation. The general morphology and the cytochemical reaction of marker enzymes for mitochondria and Golgi complexes were investigated. The results were summarized as follows 1. DMSO induced elongation and narrowing of the cells and increase of mitochondrial reaction in myocardial cells. 2. DMSO induced destruction and disruption of myofibrils in myocardial cells resulting in increase of contractile activities. 3. In the endothelial cells, DMSO suppressed proliferative activities but thiamine pyrophosphatase reactions were enhanced indicating increase of Golgi complex activity. 4. DMSO seemed to hamper with the adhesiveness and motility of the endothelial cells causing the decrease of the number of cells in vitro.

Effects of Trypsin, Collagenase and Dimethyl Sulfoxide on Dissociation of Rat Heart Cells.: Chang Woo Park, Yung Chang Lee; Yeungnam Univ J Med. 1987;4(1):17-23. Published online August 31, 1987; DOI: https://doi.org/10.12701/yujm.1987.4.1.17

Abstract PDF: New born rat heart cells were dissociated using trypsin and/or collagenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.

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